杭州浙大迪迅生物基因工程有限公司JACI：组份诊断在螨虫过敏诊断中的临床价值
发布日期：2019-01-02
原标题：螨虫过敏分子组份诊断的临床价值
延伸阅读
JACI：
DOI: https://doi.org/10.1016/j.jaci.2011.12.661
Abstract:
Rationale
In patients allergic to mites IgE antibody test with a natural allergen extract does not reveal the nature of the sensitizing molecules. Polisensitization to House Dust Mites (HDM) and Storage Mites (SM) is frequently observed. The aim of this study is to define whether determination of molecular patterns of sensitization is useful for optimizing the diagnosis of these patients.
Methods
101 patients attending our outpatient clinic suffering rhinoconjunctivitis and/or asthma sensitized to mites were included. Skin prick test (SPT,) and specific IgE (ImmunoCAP) to the HDM Dermatophaghoides pteronyssinus and to the SM Lepidoglyphus destructor were done. In parallel, IgE to a panel of allergen components including Der p1, Der p2, Der f1, Der f2, Lep d2 and Der p10 (mite tropomyosin) were performed in the Advia Centaur platform.
Results
All subjects showed positive SPT and 99% positive IgE to D. pteronyssinus. In these patients 81.2% sensitization to Der p1 and 93.1% to Der p2 was found. SPT and specific IgE to L. destructor was positive in 84.2% and 85.1% respectively, although only 42.6% had sensitization to Lep d2. Positivity to Derp 10 was found in only 2% of patients.
Conclusions
In mite allergy the molecular diagnosis does not always coincide with the diagnosis performed with natural extracts, especially in SM. We consider important to know the molecular components responsible for the sensitization, in order to provide a more accurate diagnosis and etiological treatment to our patients.
All Author:
F. Rodriguez Fernandez, M. Lopez Hoyos, P. Munoz Cacho, C. Barbeitio, G. Perdomo Gutierrez, A. Galan Nieto, M. Arina Sanche
——来自浙大迪迅
①在螨虫过敏的患者中，用天然过敏原提取物进行IgE抗体检测并不能揭示致敏分子的性质。屋尘螨(HDM)和仓储螨(SM)的致敏作用较为常见。本研究的目的是确定致敏的分子模式是否有助于优化对这些病人的诊断。②101名在我们门诊就诊的对螨虫敏感的鼻结膜炎及/或哮喘病人被纳入本研究。对所有病人分别进行屋尘螨和害嗜鳞螨提取液的皮肤点刺试（SPT， ALK-Abelló）和特异性IgE (ImmunoCAP)。同时，在Advia Centaur平台上，检测包括Der p1、Der p2、Der f1、Der f2、Lep d2和Der p10（螨虫原肌球蛋白）在内的过敏原组分特异性IgE。③所有受试者对屋尘螨SPT阳性，99%的受试者的屋尘螨特异性IgE阳性。这些患者对Der p1的敏感性为81.2%，对Der p2的敏感性为93.1%。SPT和特异性IgE对害嗜鳞螨的敏感性分别为84.2%和85.1%，对Lep d2的敏感性仅为42.6%。只有2%的患者对derp10呈阳性反应。④在螨虫过敏中，分子诊断并不总是与天然提取物的诊断一致，尤其是在仓储螨中。我们认为重要的是了解致敏的分子组份，以便为患者提供更准确的诊断和病因治疗。延伸阅读
JACI：
[IF:13.1]
Clinical Usefulness Of Diagnosis By Molecular Components In Mite AllergyDOI: https://doi.org/10.1016/j.jaci.2011.12.661
Abstract:
Rationale
In patients allergic to mites IgE antibody test with a natural allergen extract does not reveal the nature of the sensitizing molecules. Polisensitization to House Dust Mites (HDM) and Storage Mites (SM) is frequently observed. The aim of this study is to define whether determination of molecular patterns of sensitization is useful for optimizing the diagnosis of these patients.
Methods
101 patients attending our outpatient clinic suffering rhinoconjunctivitis and/or asthma sensitized to mites were included. Skin prick test (SPT,) and specific IgE (ImmunoCAP) to the HDM Dermatophaghoides pteronyssinus and to the SM Lepidoglyphus destructor were done. In parallel, IgE to a panel of allergen components including Der p1, Der p2, Der f1, Der f2, Lep d2 and Der p10 (mite tropomyosin) were performed in the Advia Centaur platform.
Results
All subjects showed positive SPT and 99% positive IgE to D. pteronyssinus. In these patients 81.2% sensitization to Der p1 and 93.1% to Der p2 was found. SPT and specific IgE to L. destructor was positive in 84.2% and 85.1% respectively, although only 42.6% had sensitization to Lep d2. Positivity to Derp 10 was found in only 2% of patients.
Conclusions
In mite allergy the molecular diagnosis does not always coincide with the diagnosis performed with natural extracts, especially in SM. We consider important to know the molecular components responsible for the sensitization, in order to provide a more accurate diagnosis and etiological treatment to our patients.
All Author:
F. Rodriguez Fernandez, M. Lopez Hoyos, P. Munoz Cacho, C. Barbeitio, G. Perdomo Gutierrez, A. Galan Nieto, M. Arina Sanche
2018-12-24 Article
创建过敏性疾病的科研、科普知识交流平台，为过敏患者提供专业诊断、治疗、预防的共享平台。
