杭州浙大迪迅生物基因工程有限公司Release date：2018-11-13
World Allergy Organization journal
DOI:org/10.1186/1939-4551-6-S1-P129
Abstract:
Background:Shellfish allergy is one of the commonest food allergies in the tropics.
Methods:Serum allergen-specific IgE of 105 subjects were quantified using ImmunoCAP and ImmunoCAP ISAC biochips. The subjects were classified based on a convincing clinical history and food challenge testing (FC) (dose=70g) to Penaeus monodon and Litopenaeus vannamei. Group1A: Either SA with FC positive to either shrimp (n =22) or SA admitted to emergency departments for severe reactions but no FC performed (n=14) (total n=36); and Group1B: Reported SA with FC negative (n=31). Group2: Shellfish tolerant DM sensitized controls (n= 38).
Results:All 105 subjects but one were sensitized to at least one of 3 DM tested (Dermatophagoides pteronyssinus, Dermatophagoides farina, Blomia tropicalis), with highest sensitization rates to Blo t 5 followed by Der f 1. DM and shrimp tropomyosin showed high correlation (p<0.001). Group1A had higher rates of sensitization to tropomyosins compared to Group1B (Der p 10 [33.3%vs9.7%], and Pen m 1 [33.3%vs9.7%], p<0.037); and to Group2 (Blo t 10 [19.4%vs0%], Pen m 1 [33.3%vs5.3%], Pen I 1 [27.8%vs5.3%], Lit v 1 [22.2%vs5.3%], p<0.05). Sensitization to Lit v 2 were higher in Group1A (22.2%) compared to Group1B (6.5%) and Group2 (5.2%) (p<0.093). The sensitization rates to Lit v 3, 4 were low (<10%). A positive test for a combination of shrimp (ImmunoCAP f24)+any tropomyosin+any shrimp allergens gave the highest sensitivity(81.8%) to distinguish FC positive from negative subjects but had a low specificity of 24.1%. The specificity was highest (93.1%) when using a positive test for Der p 10 or any shrimp tropomyosin, but sensitivity was low (31.8%).
Conclusions:Tropomyosins are highly cross reactive across species and is significantly associated with SA in the tropics. ISAC Immunocap improved the accuracy to detect FC+ve SA.
All Authors:
Irenaeus Paul Chia
Correspondence:
National University of Singapore, Singapore
All Authors:
Irenaeus Paul Chia, Gaik Chin Yap, Bee Wah Lee, Hugo Van Bever, Lynette Shek Pei-Shi, Irvin Francis A Gerez, Genevieve Llanora, Yew Kuang Cheng, Bernard Thong, MA Curotto De Lafaille, Chwee Ying Tang
[IF:5.676]
Food allergy and anaphylaxis – 2044. Component resolved allergen sensitzation profiles in shrimp allergy in the tropicsDOI:org/10.1186/1939-4551-6-S1-P129
Abstract:
Background:Shellfish allergy is one of the commonest food allergies in the tropics.
Methods:Serum allergen-specific IgE of 105 subjects were quantified using ImmunoCAP and ImmunoCAP ISAC biochips. The subjects were classified based on a convincing clinical history and food challenge testing (FC) (dose=70g) to Penaeus monodon and Litopenaeus vannamei. Group1A: Either SA with FC positive to either shrimp (n =22) or SA admitted to emergency departments for severe reactions but no FC performed (n=14) (total n=36); and Group1B: Reported SA with FC negative (n=31). Group2: Shellfish tolerant DM sensitized controls (n= 38).
Results:All 105 subjects but one were sensitized to at least one of 3 DM tested (Dermatophagoides pteronyssinus, Dermatophagoides farina, Blomia tropicalis), with highest sensitization rates to Blo t 5 followed by Der f 1. DM and shrimp tropomyosin showed high correlation (p<0.001). Group1A had higher rates of sensitization to tropomyosins compared to Group1B (Der p 10 [33.3%vs9.7%], and Pen m 1 [33.3%vs9.7%], p<0.037); and to Group2 (Blo t 10 [19.4%vs0%], Pen m 1 [33.3%vs5.3%], Pen I 1 [27.8%vs5.3%], Lit v 1 [22.2%vs5.3%], p<0.05). Sensitization to Lit v 2 were higher in Group1A (22.2%) compared to Group1B (6.5%) and Group2 (5.2%) (p<0.093). The sensitization rates to Lit v 3, 4 were low (<10%). A positive test for a combination of shrimp (ImmunoCAP f24)+any tropomyosin+any shrimp allergens gave the highest sensitivity(81.8%) to distinguish FC positive from negative subjects but had a low specificity of 24.1%. The specificity was highest (93.1%) when using a positive test for Der p 10 or any shrimp tropomyosin, but sensitivity was low (31.8%).
Conclusions:Tropomyosins are highly cross reactive across species and is significantly associated with SA in the tropics. ISAC Immunocap improved the accuracy to detect FC+ve SA.
All Authors:
Irenaeus Paul Chia
Correspondence:
National University of Singapore, Singapore
All Authors:
Irenaeus Paul Chia, Gaik Chin Yap, Bee Wah Lee, Hugo Van Bever, Lynette Shek Pei-Shi, Irvin Francis A Gerez, Genevieve Llanora, Yew Kuang Cheng, Bernard Thong, MA Curotto De Lafaille, Chwee Ying Tang
2018-10-24 Meeting abstract
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