杭州浙大迪迅生物基因工程有限公司Release date：2018-12-24
JACI:
DOI: https://doi.org/10.1016/j.jaci.2006.11.466
Abstract:
Abstract
RATIONALE: Peanut is an important food allergen because of its clinical severity and increasing prevalence, especially in some western countries. However few data came from China.
METHODS: A careful history; skin prick test for peanut; specific IgE for peanut (ImmunoCAP); peanut challenge test, SDS-PAGE electrophoresis for peanut extract, immunoblot.
RESULTS: Eight subjects had the mean age 24 ranging from 3 to 64 years old. Fourteen protein bands were identified in the peanut extraction, with five protein abundant bands at 63 kd, 41.7 kd, 39 kd, 34.8 kd, and 17.9 kd. At least eight bands were identified by the allergic sera. These allergenic proteins had molecular weights of 17.9 kd (recognized by 4/8 sera), 23.5 kd (recognized by 4/8 sera), 41.7 kd (recognized by 4/8 sera), 48.5 kd (recognized by 5/8 sera), 63 kd (recognized by 4/8 sera), 34.8 kd (recognized by 1/8 sera), 39 kd (recognized by 3/8 sera), 53 kd (recognized by 2/8 sera).respectively.
CONCLUSIONS: Patients showed individual difference in view of reaction to the peanut allergenic components
Author:
H. Li
[IF:13.1]
Analysis of Peanut Allergenic Components for Chinese PatientsDOI: https://doi.org/10.1016/j.jaci.2006.11.466
Abstract:
Abstract
RATIONALE: Peanut is an important food allergen because of its clinical severity and increasing prevalence, especially in some western countries. However few data came from China.
METHODS: A careful history; skin prick test for peanut; specific IgE for peanut (ImmunoCAP); peanut challenge test, SDS-PAGE electrophoresis for peanut extract, immunoblot.
RESULTS: Eight subjects had the mean age 24 ranging from 3 to 64 years old. Fourteen protein bands were identified in the peanut extraction, with five protein abundant bands at 63 kd, 41.7 kd, 39 kd, 34.8 kd, and 17.9 kd. At least eight bands were identified by the allergic sera. These allergenic proteins had molecular weights of 17.9 kd (recognized by 4/8 sera), 23.5 kd (recognized by 4/8 sera), 41.7 kd (recognized by 4/8 sera), 48.5 kd (recognized by 5/8 sera), 63 kd (recognized by 4/8 sera), 34.8 kd (recognized by 1/8 sera), 39 kd (recognized by 3/8 sera), 53 kd (recognized by 2/8 sera).respectively.
CONCLUSIONS: Patients showed individual difference in view of reaction to the peanut allergenic components
Author:
H. Li
2018-12-21 Article
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